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      Beckman貝克曼 純化磁珠 核酸純化試劑450ml(A63882)

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      Beckman貝克曼 核酸純化試劑450ml(A63882)純化磁珠

       

       

       

       

      Beckman貝克曼 純化磁珠 核酸純化試劑450ml(A63882)

       

       

       

       

       

       

       

      1雙鏈和單鏈DNA都能得到純化


      2、有效去除dNTP、引物、引物二聚體、鹽離子和其它雜質


      2、200bp以上的核酸回收率高達90%以上,核酸長度最小要求100bp


      3、純化后的產物在4℃溫度下保持7天不被降解


      4、適用于PCR體系純化、酶切和連接反應體系純化

       

      Application: PCR purification

      Downstream Application Techniques: PCR, genotyping (SNP detection) fragment analysis, sequencing (Sanger and Next Generation), cloning, primer walking

      Agencourt AMPure XP Features

      • High recovery of amplicons, greater than 100 bp
      • Efficient removal of unincorporated dNTPs, primers, primer dimers, salts and other contaminants
      • Stable PCR products post-cleanup: No PCR degradation after storage at 4° C for seven days
      • Efficient recovery of double stranded and single stranded DNA templates
      • Consistent recovery throughout the kit’s 12 month shelf life
      • Faster manual and automated processing as compared to traditional post-PCR cleanup methods

      Process Overview

      Agencourt AMPure XP process overview

      1. PCR reaction 2. Binding of PCR amplicons to magnetic beads 3. Separation of PCR amplicons bound to magnetic beads from contaminants 4. Washing of PCR amplicons with Ethanol 5. Elution of PCR amplicons from the magnetic particles 6. Transfer away from the beads into a new plate

      High Recovery

      Agencourt AMPure XP purification system recovers amplicons greater than 100 bp with consistency and high reproducibility. The recovery efficiency of the Agencourt AMPure XP kit for small and large amplicon sizes is superior to the traditional filtration PCR cleanup methods (Figure 1).

      Agencourt AMPure XP process figure

      Figure 1. The recovery of the PCR products post-PCR cleanup was measured by spectrophotometry; 200 bp, 800 bp and 10 Kb PCR products where purified with the Agencourt AMPure XP kit, QIAquick* kit and QuickStep* filtration system.

      Walk-away Automated Solution

      Agencourt AMPure XP post-PCR cleanup has been automated on three Beckman Coulter liquid handlers, the Biomek 3000, NXP and FXP for processing scalability. The LIMS compatible automation functionality is aided by a user-friendly interface equipped with a quick startup menu, a deck setup walk-through menu, a method recovery menu, and a procedure menu.

      Ordering Information

      Product Size Kit Components

      Product #

      Agencourt AMPure XP 5 mL Kit 139/278 preps1 Agencourt AMPure XP Reagent

      A63880

      Agencourt AMPure XP 60 mL Kit 1,666/3,333 preps1 Agencourt AMPure XP Reagent

      A63881

      Agencourt AMPure XP 450 mL Kit 12,500/25,000 preps1 Agencourt AMPure XP Reagent

       

       

        

       

       

       

       

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